Introduction and Aims: H. PYLORI has been implicated in peptic diseases, some with detrimental consequences such as ulcer or cancer. Since considerable genetic heterogeneity has been observed within H. PYLORI population worldwide, it appears an ideal achievement to recruit PCR-based methods and design genetic markers which recognize isolates from normal and symptomatic individuals. In this study 61 H. PYLORI isolates from dyspeptic patients were fingerprinted by REP-PCR.Materials and Methods: REP-PCR was performed on extracted DNAs of 61 H. PYLORI isolates from 39 normal, 18 ulcer and 4 cancer patients. Synthetic 18-nt primers, specific for interspersed repetitive elements in the bacterial genome, were recruited. PCR conditions were optimized and reproducibility of the reactions were confirmed. The size and number of PCR products were determined and DNA fingerprints of all isolates were analyzed by NTSYSpc programme, and dendrograms were generated.Results: Among 39 H. PYLORI isolates from normal patients 28 comprised a distinct cluster and 5 clustered along with isolates from ulcer patients. The remaining 6 isolates comprised a separate cluster distinct from other groups. Among 18 isolates from ulcer patients, 17 classified in a specific cluster, only one isolate was clustered along with isolates from normal patients. Isolates from cancer patients consisted a quite distinct cluster.Conclusions: In this study REP-PCR was used to show that majority of isolates from normal, ulcer, and cancer patients have distinct fingerprints which can be recruited for predicting the outcome of the infection with certain H. PYLORI isolates. It is concluded that REP-PCR is an effective and reproducible technique for fingerprinting H. PYLORI isolates from different human origins.

HELICOBACTER PYLORI colonize in gastric mucosa and causes disorders in upper gastro intestine. The clinical outcome of HELICOBACTER PYLORI infection may be associated with or without Cag A or ice A genes.To investigate presence of cag A and ice A genes in HELICOBACTER PYLORI strains among patients with gastritis, Gastric ulcer and duodenal ulcer, 80 gastric and duodenal biopsy specimens were taken. Urease test was perfomed for all specimens that H.PYLORI strains were isolated from 25 patients. We Perfomed DNA extraction used allelic specific PCR to examine iceA and Cag A status of H.PYLORI isolates. H. PYLORI was isolated in 25 cases. The PCR method indicates prevalence of iceA gene in strains isolated from patients with gastritis, gastric ulcer and duodenal ulcer was 25%, 50% and 25% respectively. Additionally iceA1 gene was found in 75% of the isolates strains and it was predominant. Prevalence of cagA genes in strains isolated from patients with gastritis, Gastric ulcer and duodenal ulcer was 36/36%, 50% and 16/16% respectively. According to the finding it is suggested the prescense of cagA gene in strains of HELICOBACTER PYLORI may play an important role in severity disorders or peptic ulcer. But in conclusion we cannot be suggested any of these gene tobe an determining the nature of clinical disease byH. PYLORI infection, Various geographiced region,may be associated with different frequency in cagA and iceA.

Iranian men are at risk of developing gastrointestinal cancer caused by H. PYLORI. It is very imperative to find effective methods to control this bacterium as there are currently no very effective treatments for it. Honey has been shown to have antimicrobial properties against various pathogens. This study analyzed 15 honey samples from A. florea bees, collected from different floral and geographical origins, for their antimicrobial efficacy against H. PYLORI. Using atomic absorption measurements, the honey samples were also tested for their phenolic and flavonoid content, protein concentration, and mineral content. Antioxidant activity was determined using the FRAP, DPPH, and ABTS methods. The antibacterial activity of honey samples was investigated both in-vitro and in-vivo in the gastrointestinal tract of mice. Statistical analysis revealed a significant positive correlation between antioxidant activity and antibacterial activity. All honey samples showed antimicrobial activity in-vitro, among which jujube honey from Bushehr exhibiting the highest activity. Differences in antioxidant and antimicrobial activities were likely due to the flora of the plants and the geographic region from which the honey was harvested. Based on these results, A. florea honey may be used in the prevention and treatment of H. PYLORI-associated infections and inflammation of the gastrointestinal tract. This feature can be applied to the control of HELICOBACTER PYLORI along with other available measures.